Azotobacter Biofertilizers


In the preset context, the best alternative of chemical fertilizer is necessary because of its adverse effects on the soil health. There are several alternatives available to enhance the soil fertility one of them is Azotobacter. It is a free–living N2– fixer diazotroph that has several beneficial effects on the crop growth and yield. It helps in synthesis of growth regulating substances like auxins, cytokinin and Giberellic Acid (GA). In addition, it stimulates rhizospheric microbes, protects the plants from phyto–pathogens, improves nutrient uptake and ultimately boost up biological nitrogen fixation. The abundance of these bacteria in soil is related to many factors, mostly soil pH and fertility.

Azotobacter spp. are Gram negative, free-living, aerobic soil dwelling, oval or spherical bacteria that form thick-walled cysts (means of asexual reproduction under favorable condition). There are around six species in the genus Azotobacter some of which are motile by means of peritrichous flagella, others are not. They are typically polymorphic and their size ranges from 2-10 µm long and 1-2 µm wide. The Azotobacter genus was discovered in 1901 by Dutch microbiologist and botanist Beijerinck et al. (founder of environmental microbiology). A chroococcum is the first aerobic free-living nitrogen fixer.

Leaf Area Index (LAI)

Azotobacterin balanced nutrient condition results in 3.5 % increment in LAI at rosette stage of canola crop and additional application of Azotobactershot up the yield by 21.17 % over the control (chemical fertilizers). The rate of increase in the leaf area determines the photosynthetic capacity of plant, which leads to better assimilation of produce and towards yield. Using Azotobacter spp. potato yield has been increased by 33.3% and 38.3%.  Similarly 20% yield increment is recorded in Azotobacter inoculated plants  reported that, number of branches, pod per plant and 1000 grain weight also increased with Azotobacter application.

Biochemical effects

Several strains of Azotobacter are capable of producing amino acids when grown in culture media amended with different carbon and nitrogen sources. Substance like amino acid produced by these rhizobacteria are involved in many processes that explain plant-grown promotion. Biochemical analysis of chlorophyll, nitrogen, phosphorous, potassium and protein content was higher in Azotobacter inoculated plants as compared to non-inoculated control plants.

Anti-pathogenic response

Azotobacter spp. are capable to produce siderophore, they bind to the available form of iron Fe+3 in the rhizosphere, thus making it unavailable to the phytopathogens and protecting the plant health; similarly Hydro Cyanine (HCN) production was higher in traits of Azotobacter (77.00 %). Azotobacter secretes an antibiotic with a structure similar to anisomycin, which is a documented fungicidal antibiotic. Azotobacter, in sufficient numbers, will out-compete pathogens for food. Some of the pathogens that have been controlled by Azotobacterin the soil and on the leaf

Effects of chemical fertilizer in Azotobacter

Combined application of bio-fertilizer with 50% of chemical fertilizers (N and P) has significant effect in plant growth, plant height, number of branches, fresh and dry weight of safflower in comparison with chemical fertilizers alone. Similarly, application of Azotobacter biophosphate and organic fertilizers, with half dose of chemical fertilizers increase the economic yield of safflower. Efficiency of Azotobacter found decreased with increased N level.The best combination was recorded with NH4Cl at 0.1 g/L whereas, action of copper in Azotobacter found toxic even in very low concentration.The population of Azotobacter may suffer due to high amount of nitrates and the acidic environment created because of chemical fertilizer.

Effect of pesticides in Azotobacter

Balajee & Mahadevanreported that, the effect of herbicide 2, 4-D and its products; p-chlorophenoxy-acetic acid and p-chlorophenol were utilized by A. croococcum as carbon source, which ultimately stimulate nitrogenase enzyme. Similar result found by Kanungo et al. which revealed insecticide carbofuran also stimulates nitrogenase enzyme activity. Martinez et al. found that, herbicide simazine have no effect on A. chroococcum growth either on standard medium or on dialysed soil and sterilized soil medium. The presence of 50-300 mg of simazine per ml of culture or in one gram of soil did have a stimulating effect on Azotobacter. When Azotobacter is grown in presence of simazine, the cells have a higher ATP content than the control. Whereas organophosphorous insecticides profenofos and chloropyrifos had adverse effect on the number of aerobic nitrogen fixers and decreased nitrogen fixation.

Azotobacter in nutrient cycling

Azotobactermakes availability of certain nutrients like Carbon, Nitrogen, Phosphorus and Sulphur through accelerating the mineralization of organic residues in soil and avoid uptake of heavy metals.Azotobacter can be an important alternative of chemical fertilizer because it provides nitrogen in the form of ammonia, nitrate and amino acids without situation of over dosage, which might be one of the possible alternatives of inorganic nitrogen source (eg. Urea). It also helps to sustain the plant growth and yield even in case of low phosphate content soil, as well as helps in uptake of macro and certain micro nutrients which facilitates better utilization of plant root exudates itself.

Role of Azotobacter in soil fertility

Azotobacter in soil: The presence of Azotobacter sp. in soils has beneficial effects on plants, but the abundance of these bacteria is related to many factors, soil physico-chemical (e.g. organic matter, pH, temperature, soil moisture) and microbiological properties. Its abundance varies as per the depth of the soil profile. Azotobacteria are much more abundant in the rhizosphere of plants than in the surrounding soil and that this abundance depends on the crop species.

Nitrogen fixation: Nitrogen is the component of protein and nucleic acids and chlorophyll. Thus, nitrogen supply to the plant will influence the amount of protein, amino acids, protoplasm and chlorophyll formed. Therefore, adequate supply of nitrogen is necessary to achieve high yield potential in crop.

The atmosphere comprises of ~78% nitrogen as an inert, in unavailable form. Above every hectare of ground there are ~80000 tones of this unavailable nitrogen. In order to be converted to available form it needs to be fixed through either the industrial process or through Biological Nitrogen Fixation (BNF). Without these nitrogen-fixers, life on this planet may be difficult.

Nitrogen (N) deficiency is frequently a major limiting factor for crops production. Nitrogen is an essential plant nutrient, widely applied as N-fertilizer to improve yield of agriculturally important crops. An interesting alternative to avoid or reduce the use of N-fertilizers could be the exploitation of Plant Growth-Promoting Bacteria (PGPB) capable of enhancing growth and yield of many plant species, several of agronomic and ecological significance.

Azotobacterspp. are non-symbiotic heterotrophic bacteria capable of fixing an average 20 kg N/ha/per year.Bacterization helps to improve plant growth and to increase soil nitrogen through nitrogen fixation by utilizing carbon for its metabolism.

Seed inoculationAzotobacterand nutrient uptake

Seed Inoculated with Azotobacter helps in uptake of N, P along with micronutrients like Fe and Zn, in wheat, these strains can potentially be used to improve wheat nutrition. Seed inoculation of Azotobacter profoundly contribute to increase yield by supplying nitrogen to the crops. Inoculation of seeds with Azotobacter chroococcumincreased carbohydrate and protein content of two corn varieties (Inra210 and Inra260) in greenhouse experiment. There is increment in Maize biomass with the application of manure and Azotobacter.

In nitrogen-deficient sand, seed inoculation increased plant length, dry weight, and nitrogen content in addition to a significant increase in soil nitrogen. It was found that A. chroococcumat concentration of 108 cfu ml-1 increased seed germination of Cucumber.Seeds of wheat (Triticum aestivum) were inoculated with 11 bacterial strains of A.chroococcum, Research result showed that all A. chroococcums trains had positive effect on the yield and N concentrations of wheat.

Role of Azotobacter in growth substances production and promotion

Besides, nitrogen fixation, Azotobacter produces, Thiomin, Riboflavin, Nicotin, Indol Acetic Acid and Giberalin. When Azotobacter is applied to seeds, seed germination is improved to a considerable extent. Brakel & Hilger showed that Azotobacter produced Indol-3-Acetic Acid (IAA) when tryptophan was added to the medium. Hennequin & Blachere found only small amounts of IAA in old cultures of Azotobacter to which no tryptophan was added.

Bacteria of the genus Azotobacter synthesize auxins, cytokinins, and GA-like substances, and these growth materials are the primary substance controlling the enhanced growth of tomato. These hormonal substances, which originate from the rhizosphere or root surface, affect the growth of the closely associated higher plants. Eklund et al demonstrated that the presence of Azotobacter chroococcum in the rhizosphere of tomato and cucumber is correlated with increased germination and growth of seedlings. Puertas & Gonzales report that dry weight of tomato plants inoculated with Azotobacter chroococcum and grown in phosphate-deficient soil was significantly greater than that of non inoculated plants. Phytohormones (auxin, cytokinin, gibberellin) can stimulate root development.

High Gibberillic acid production was detected in Azotobacter(71.42 %) isolates. Higher phosphate solubilization was detected in the isolates of Azotobacter (74.28 %) followed by Pseudomonas (63.00 %). Gibberellins applied in small quantities to the soil or rosettes on the leaves and shafts of certain plant produces an increase in height. In grains such as wheat and corn, they also cause an increase in length of the leaves. In some cases they also increase the fresh weight and dry weight. However it doesn’t produce any effect on the growth of the roots.

Brown & Burlinghamhave found that after treating tomato seeds or seedling roots with small amounts (0.5-0-01 pg.) of commercially produced gibberellins GA 3the plants responded in the same way as after treatments with 14-day cultures of Azotobacter chroococcumstrain A 6Azotobacter chroococcumstrains isolated from the sugar beet rhizosphere were also shown to produce gibberelins; the growth of pea hypocotyl was equivalent to a GA3 concentration of 0.003-0.1 μg/cm3 culture.  Similarly, Cytokinins are related to nucleic acids with precursor substances that act to stimulate cell division in vegetative growth areas.

These responses suggest that Azotobacter probably influences the development of plants by producing growth-regulating substances. Therefore, Azotobacter spp. is often regarded as a member of “Plant Growth Promoting Rhizobacteria (PGPR)”.

It was also concluded that Azotobacterinoculants have a significant promoting effect on growth parameters like root, shoot length and dry mass of bamboo and maize seedlings in vitroand in pot experiments. The dual inoculation of A. chroococcumand P. indica had beneficiary response on shoot length, root length, fresh shoot and root weight, dry shoot and root weight, and panicle number that affect growth of rice plant.

Adathoda vasicaplants inoculated with A. chroococcumrevealed significantly increased root nitrogen content compared to the control plants. The Azotobacters which were inoculated with Rhizophora seedlings, increased significantly the average root biomass up to by 98.2%, the root length by 48.45%, the leaf area by 277.86%, the shoot biomass by 29.49% as compared to controls and they also increased the levels of total chlorophylls and carotenoids up to by 151.0% and 158.73%, respectively. The Azotobacter inoculated crop show better growth rate. During rosette stage of canola the Crop Growth Rate (CGR) found highest i.e. 10-12 % increment.

There is increment in dry matter accumulation in Azotobacter inoculated plants; it stimulates development of foliage, roots, branching, flowering and fruiting which is triggered by fixed nitrogen and plant growth regulator like substance produced. It also increases plant tolerance to lack of water under adverse condition. Similar result put forwarded by Sandeep et al. which revealed that there is better growth response of Azotobacter inoculated plants as compared to non-inoculated control plants. Better crop growth response ultimately results in better dry matter accumulation.